Botnical ID by HPTLC and HPLC
Identity Testing of Botnicals
What is HPTLC?
High Performance Thin Layer Chromatography (HPTLC) is not the conventional TLC of the past, but is a modern technique involving sophisticated instrumentation, standardized and documented procedures, as well as validated methods. This allows the user to achieve reproducible results in a cGMP environment, and its proper implementation means that WHEN your lab is inspected by the FDA it can meet the requirements of the 100% ID rule.
Other techniques can address identification only in part. They either are limited to plant parts (microscopy), have difficulties with natural variability of botanical materials (IR techniques), or focus on quantitative comparison of separated markers (HPLC). All other techniques lack the versatility and flexibility of HPTLC for the analysis of botanicals. HPTLC is the ONLY single technique that offers a generally applicable approach to compliance with the FDA requirement of 100% botanical identification.
Why HPTLC Identification Required?
The fundamental question of identity is at the heart of regulatory compliance and offering safe and effective botanical products. Plant chemistry is intricate, and today's industrial methods make it even more so. The use of the incorrect plant or plant part is all too typical in adulteration. Botanical identification is crucial, which includes confirming the botanical genus, species, and plant component. Many plants have close cousins with similar appearances and scents. They have comparable actions at times, and very distinct actions at other times; occasionally, one is harmless and effective, while the other is hazardous. Because your product label must be precise and correct, you must know the genus and species of any plant you intend to sell.
From the viewpoint of marker compounds, High Performance Liquid Chromatography (HPLC) Fingerprinting is a helpful and complementary method used to evaluate the identity and quality of complex herbal blends and different ingredients. Chromatographic fingerprints provide data that aid in production control and guarantee batch-to-batch consistency.