UV-Visible Spectroscopy, also known as Ultraviolet-Visible Spectroscopy (UV-Vis), is a general and widely utilized analytical technique in scientific research and industrial laboratories. It measures the absorbance or transmission of a substance at specific UV and visible wavelengths and produces valuable information for quantitative and qualitative analysis.

At Chromak Research, a top contract testing lab, we provide UV-Vis spectroscopy testing for various products from several industries including pharmaceuticals, chemicals, cosmetics, food, agriculture, microbiology, and life science. We adhere strictly to the USP <857> guidelines for attaining accuracy, consistency, and regulatory compliance for each UV-Vis analysis.

What is UV-Visible Spectroscopy?

UV-Visible Spectroscopy is a quantitative analysis technique that measures the quantity of ultraviolet and visible light absorbed by an analyte at specific wavelengths. The quantity of light absorbed is linear with respect to the concentration of analytes within the sample, and UV-Vis spectroscopy is thus an extremely good analysis technique for quantitative analyses.

This technique is particularly applicable for:

Measuring the concentration of compounds in a solution.

Analyzing impurities and contaminants in raw materials or products.

Tracking chemical reactions and stability over time.

Inventing and calibrating analytical procedures for novel formulations.

How UV-Visible Spectroscopy Functions: Lambert-Beer’s Law

The basic mechanism of UV-Visible Spectroscopy is dictated by Lambert-Beer’s Law, which is:

A = ε × b × c

Where:

A = Absorbance

ε = Molar absorptivity (constant that depends on the substance)

b = Path length of the sample cell (typically in cm)

c = Concentration of the analyte

Such a relationship allows accurate determination of the concentration of an analyte in a sample by analyzing absorbance at a particular wavelength. Absorbance vs. concentration is central to calibration curve establishment, assay, and method validation.

Applications of UV-Vis Spectroscopy Across Industries

Chromak Research offers analytical testing services by UV-Vis spectroscopy across various industries:

API assay, dissolution studies, and impurity profiling.

Raw chemicals and intermediate concentration analysis and quality control.

UV absorber analysis in sunscreens, colorimetry, and stability testing.

Food colorant, vitamins, and nutrient testing.

Enzyme activity analysis, protein and nucleic acid analysis, cell enumeration.

Our UV-Vis testing ensures your products are compliant with the regulations and have specifications of high quality.

Why Choose Chromak Research for UV-Vis Spectroscopy

At Chromak Research, we pair experience with the latest technology to provide you with reliable analytical test services. Here’s why companies in every industry rely on us:

Tailored Method Development

Our scientists are trained to develop and qualify UV-Vis methods appropriate for your unique product or process requirements.

Affordable Services

We focus on obtaining top-quality results at the lowest cost rate possible in order to make analytical testing a reality for companies of all sizes.

Quick Turnaround Time

Our streamlined process has prompt reporting, so you can meet deadlines.

Regulatory Compliance

We have standard industry procedures established and offer documentation to support regulation compliance for quality and safety.

Partner with Chromak Research

Regardless of whether you require your samples to be analyzed for UV-Vis spectroscopy, your best option is Chromak Research. Our highly trained staff are at the ready to aid you in analyzing your samples, developing new protocols, or support your R&D and quality control programs. If you’re in the pharmaceutical, chemical, cosmetic, food, or biotech industries, you can be sure that we are at your service to ensure your product’s purity and quality.

Contact us today to learn more about our UV-Visible Spectroscopy services or to receive a quote for your upcoming testing project.