Enzyme Activity Assay
Enzymes are relatively sensitive to environmental and manufacturing processes, including temperature, moisture and interactions with other ingredients. It is important to verify the actual enzyme activity in the final product, rather than relying on the formulated concentration. As functional bio active molecules, enzymes may experience degradation or loss of activity during their storage. It is therefore very important that the appropriate analytical method is used. It is also a special characteristic of enzymes that their optimal working conditions (e.g. pH, temperature, metals, cofactors, and specific substrates) vary depending on the source and type of enzyme.
Chromak Research, Inc offers quantitative analysis of enzyme levels in a variety of matrices ranging from industrial fermentation concentrated preparations, to low levels in food ingredients such as spices, flour and Dietary supplements. Chromak Research, Inc offers the following routine assays from the Food Chemicals Codex (FCC), United States Pharmacopoeia (USP), Association of Analytical Communities (AOAC), American Association of Cereal Chemists ( AACC) , Enzyme Commission (EC) methods
In order to enable our laboratory to perform the appropriate assay, please provide us with information about the type, source (or manufacturer) and range of enzymatic activity of the enzyme when possible.
| No. | ENZYMES | TESTING METHOD | BRIEF DESCRIPTION |
|---|---|---|---|
| 1 | α-Amylase (pancreatin) | USP | Starch Hydrolysis as USP |
| 2 | α-Amylase (bacterial) | FCC | Starch Hydrolysis as BAU |
| 3 | α-Amylase (nonbacterial) | FCC | Starch Hydrolysis as DU |
| 4 | Beta-Glucanase Activity | FCC | Beta Glucanase Unit (BGU) |
| 5 | Bromelain | FIP, CDU, PU or GDU method | Hydrolysis of Gelatin |
| 6 | Catalase | WM | Breaking down of hydrogen peroxide |
| 7 | Cellulase | FCC | Cellulose viscosity reduction |
| 8 | Cellulase | CMC | Cellulose DNSA colometric |
| 9 | Ceralpha Amylase | AACC #22-02.01 | Ceralpha Units |
| 10 | Chymotrypsin | FCC or USP | Hydrolysis of N-acetyl-L-Tyrosine ethyl ester |
| 11 | Diastase | FCC | Hydrolysis of Starch |
| 12 | Diastatic Activity of Honey | AOAC | Diastase Number (DN) |
| 13 | α-Galactosidase | FCC | Hydrolysis of p-Nitrophenyl a-D-galactopyranose |
| 14 | β-glucase | FCC | Hydrolysis of Lichenin |
| 15 | Glucose Oxidase Activity | FCC | Glucose Oxidative Titrimetric unit (GOTu) |
| 16 | β-Glucosidase | FCC | Hydrolysis of cellobiose |
| 17 | Hemicellulase | FCC | Hydrolysis of bean gum and monitor viscosity change |
| 18 | Invertase (Sucrase) | FCC | Hydrolysis of sucrose and optical rotation measurement |
| 19 | Lactase(Acid Beta Galactosidase)s | FCC, ALU | Using ONPG substrate |
| 20 | Lactase (Neutral Beta Galactosidase) Activity | FCC-NLU | - |
| 21 | Lipase – Pancreatin | USP | Hydrolysis of fatty acids and titration as USP |
| 22 | Lipase – Microbial | FCC, FIP | Hydrolysis of model fatty acids and titration as FIP |
| 23 | Lysozyme | FCC | Mycoccus as substrate |
| 24 | Lipoxygenase-LOX | - | oxidative coupling of (MBTH) with (DMAB) |
| 25 | Malt Diastase (Diastatic Power) | - | - |
| 26 | Milk Clotting assay | MCU | Rennin, Casein Hydrolysis |
| 27 | Nattokinase | Fibrinolytic method | Fibrinolytic activity in fibrin |
| 28 | Pancreatin (Amylase, Protease and Lipase) Activity | USP | - |
| 29 | Papain | USP, FCC | Casein hydrolysis |
| 30 | Pepsin | USP, FCC | Hemoglobin as substrate |
| 31 | Pectinase | AJDU or VPLA | Using apple juice, pectin as substrate |
| 32 | Peroxidase – POD | Methods in Enzymology and AACC Methods. | - |
| 33 | Phytase Activity (FTU) | FCC | - |
| 34 | Polyphenol oxidase PPO | AACC Method | Tyrosine Oxidation |
| 35 | Protease –Pancreatin | USP | Hydrolysis Casein as USP |
| 36 | Proteolytic Activity – Plants | FCC | Hydrolysis of Casein as PU |
| 37 | Proteolytic – Bacterial | FCC | Hydrolysis of Casein as PC |
| 38 | Proteolytic – Fungus | FCC | Hydrolysis of hemoglobin as HUT or of Casein as SAP |
| 39 | Serrapeptase Activity | JP | - |
| 40 | Superoxide Dismutase (MFU) | McCord | - |
| 41 | Trypsin | FCC, USP | Hydrolysis of N-benzoyl-L-arginin ethyl ester |
| 42 | Trypsin Inhibitor Assay | AAAC | - |
| 43 | Xylanase | Megazyme | Megazyme xylanase tablet |