The Antibiotic potency assay is a crucial test in microbiology that determines the effectiveness of antibiotics against specific bacteria. These assays help determine the concentration or activity of an antibiotic in a given sample. Chromak Research laboratory adheres to cGMP standards and possesses cutting-edge analytical instruments. Chromak Research provides a variety of services for testing Antibiotic Potency Assays followed by USP .

At Chromak Research, we offer professional consultation services throughout the entire antibiotic potency testing process. We understand that each client’s needs may vary, and we tailor our services to meet their specific requirements. Our team is available to provide guidance and support, from sample preparation and testing to result interpretation and analysis.

We perform all potency assays according to the processes set forth in USP following cGMP standards. Methodologies we use in determining antibiotic potency include the cylinder plate and the turbidimetric assays.

The USP <81> outlines the following methods for antibiotic potency assays in microbiology:

1. Cylinder-Plate Method (Agar Diffusion Method):
   • Principle: In this approach, a concentration gradient of the antibiotic is established within an agar medium by employing a cylinder or well. The antibiotic naturally disperses into the agar, resulting in the formation of an area of inhibition that hampers bacterial growth.
   • Application: Suitable for a wide range of antibiotics.
2. Turbidimetric Assay:
   • Principle: The antibiotic’s impact on a bacterial suspension is measured by assessing the decrease in turbidity (cloudiness). The extent of turbidity reduction is directly proportional to the concentration of the antibiotic.
   • Application: Suitable for assessing the potency of antibiotics that may not produce a clear zone of inhibition, particularly for bacteriostatic agents.